Pre-Owned Chromatography

FPLC Columns

Pre-owned chromatography columns for protein and nucleic acid purification. Affinity, size exclusion, ion exchange, and more - sourced from research labs worldwide.

What We Offer

Used FPLC Columns at a Fraction of New Cost

FPLC columns are among the most expensive consumables in biochemistry research. New Cytiva/GE columns can cost thousands of dollars - but well-maintained used columns perform just as well at a significantly lower price.

SIB SCI sources pre-owned chromatography columns directly from research labs, universities, and biotech companies. Each column is inspected and tested before listing. We carry affinity, size exclusion, ion exchange, and other column types from Cytiva (GE Healthcare), Bio-Rad, Tosoh, and other major manufacturers.

A

Affinity Chromatography

Highest selectivity purification. Captures target proteins directly from crude lysate with minimal steps. Essential for His-tag, GST, and antibody purification.

HisTrap HP HisTrap FF GSTrap FF Protein A NHS-activated
S

Size Exclusion (SEC)

Separates proteins by molecular size. Used for polishing steps, buffer exchange, and studying protein complexes. Critical for final-stage purity.

Superdex 75 Superdex 200 Superose 6 Sephacryl S-200 Sephacryl S-300
I

Ion Exchange (IEX)

Separates proteins by surface charge. Anion and cation exchange columns widely used for intermediate purification steps in multi-step protocols.

Q Sepharose FF SP Sepharose FF DEAE Sepharose HiTrap Q HP HiTrap SP HP
H

Hydrophobic Interaction (HIC)

Separates proteins based on surface hydrophobicity under high-salt conditions. Used as a polishing step after ion exchange or affinity chromatography.

HiTrap Phenyl HP HiTrap Butyl FF Phenyl Sepharose Octyl Sepharose
M

Mixed Mode

Combines multiple interaction modes for enhanced selectivity. Useful for difficult separations and when traditional single-mode columns are insufficient.

CaptoAdhere Capto MMC HEA HyperCel PPA HyperCel
D

Desalting & Buffer Exchange

Rapid removal of salts, small molecules, or detergents from protein samples. HiTrap Desalting and PD-10 columns for fast sample preparation.

HiTrap Desalting PD-10 HiPrep 26/10 Sephadex G-25

How It Works

Simple Process, Fast Turnaround

01

Tell Us What You Need

Send us the column type, bed volume, and application. We'll check current inventory and availability.

02

Get a Quote

We respond within 24 hours with pricing and availability. No obligation to buy.

03

Inspection & Testing

Each column is tested and inspected before shipping. We verify backpressure and document condition.

04

Worldwide Shipping

Packed properly and shipped worldwide. Columns arrive ready to use in your FPLC system.

Looking for a Specific Column?

We source chromatography columns from labs worldwide. If you don't see what you need, contact us - we respond within 24 hours.

Request a Column